Résumé
Vitis vinifera is a significant agricultural species across continents and a genomic model for perennial crops. A diversity panel of 279 cultivars from the Vassal-Montpellier Grapevine Biological Resources Centre, which represents the diversity of the three main genetics pools of this species, has served as a foundation for genome-wide association studies using genotyping-by-sequencing approaches. Part of this panel (74 cultivars) has recently been sequenced at the whole genome level. Here, we release whole-genome sequencing of the remaining 205 cultivars of the panel, using the short-read NovaSeq6000 S4 PE150 technology to achieve complete genomic coverage. To ensure consistency with prior analyses and confirm genetic identities, we performed variant calling and SNP comparison with previously published data. During this stage, we identified two mislabeled samples, which were excluded from the dataset, resulting in a final set of 72 samples from the public data. Additionally, nine representative cultivars spanning major genetic groups underwent long-read sequencing using PacBio Revio technology. All sequences have been deposited at the ENA under project PRJEB95058 for the short-read data and project PRJEB100755 for the long-reads. Variant data have been deposited in the publicly accessible GIGWA SNP database. This expanded genomic dataset establishes a comprehensive foundation for advanced genomic analyses in V. vinifera, including genome-wide association mapping, structural variant characterization, and genetic diversity assessment. The long-read sequences provide high-quality genomic resources for structural variation analysis and pangenome construction. The integration of short-and long-read sequencing technologies enhances the usefulness of this resource for understanding grapevine genomic architecture and supporting genetic improvement initiatives.