Résumé
Motivation The visualization and interpretation of evolutionary spatiotemporal scenarios is broadly and in-creasingly used in infectious disease research, ecology, or agronomy. Using probabilistic frameworks, well-known tools can infer from molecular data ancestral traits for internal nodes in a phylogeny, and numerousphylogenetic rendering tools can display such evolutionary trees. However, visualizing such ancestral informa-tion and its uncertainty on the tree remains tedious. For instance, ancestral nodes can be associated to severalgeographical annotations with close probabilities and thus, several migration or transmission scenarios exist.Results We expose a web-based tool, named AQUAPONY, that facilitates such operations. Given an evolu-tionary tree with ancestral (e.g., geographical) annotations, the user can easily control the display of ancestralinformation on the entire tree or a subtree, and can view alternative phylogeographic scenarios along a branchaccording to a chosen uncertainty threshold. AQUAPONY interactively visualizes the tree and eases the ob-jective interpretation of evolutionary scenarios. AQUAPONY’s implementation makes it highly responsive touser interaction, and instantaneously updates the tree visualizations even for large trees (which can be exportedas image files).Availability and Implementation: AQUAPONY is coded in JavaScript/HTML, available under Cecill license,and can freely used at atgc-montpellier.fr/aquapony.