Résumé
The TORC1 and PKA protein kinases are central elements of signaling networks that regulate eukary-otic cell proliferation in response to growth factors and/or nutrients. In yeast, attenuation of signaling by these kinases following nitrogen and/or carbon limitation activates the protein kinase Rim15, which orchestrates the initiation of a reversible cellular quiescence program to ensure normal chronological life span. The molecular elements linking Rim15 to distal readouts including the expression of Msn2/4-and Gis1-dependent genes involve the endosulfines Igo1/2. Here, we show that Rim15, analogous to the greatwall kinase in Xenopus, phosphorylates endo-sulfines to directly inhibit the Cdc55-protein phos-phatase 2A (PP2A Cdc55). Inhibition of PP2A Cdc55 preserves Gis1 in a phosphorylated state and consequently promotes its recruitment to and activation of transcription from promoters of specific nutrient-regulated genes. These results close a gap in our perception of and delineate a role for PP2A Cdc55 in TORC1-/PKA-mediated regulation of quiescence and chronological life span.