Résumé
Background: High random ROS production leads to generalised oxidative stress that is involved in aging and degenerative diseases, such diabetes or chronic kidney failure. The major cellular ROS sources are mitochondria and NADPH oxidase, and xanthine oxidase (XO). XO is known to be involved in ischemiareperfusion oxidative stress damages, but little is known about its possible involvement in other nutritional and physio-pathologic oxidative stress situations. Objective: The objective of this study is to determine the effect on xanthine oxidase activity in nutritional and pathological oxidative stress conditions. Methods: In this research, we investigated the contribution of XO activity in the oxidative stress situations as metabolic syndrome or chronic kidney failure. For this aim, we have determined tissue XO activity in rats subjected to different nutritional and pathological oxidative stress conditions. Results: Our results show that XO does not seem to be major contributor to the oxidative stress observed in the rats fed with high fat, or high fat/high sucrose/fructose diets. However, XO activity increased in chronic kidney failure, caused by adenine administration or by nephrectomy. Moreover, significant correlations have been observed between XO activity and some oxidative stress parameters. Conclusion: These results clarify the possible involvement of XO activity in physio-pathological oxidative stress conditions. More studies are needed in the future to determine the XO activity in other physio-pathological oxidative stress models, in order to elaborate a comprehensible view on the involvement of the XO activity in these physio-pathological oxidative stress conditions.