Which housekeeping gene is the best choice for RT-qPCR analysis in mice fed with a high-fat diet? Studies in the liver, kidney, pancreas, and intestines

Ayla Secio-Silva; Felipe Emrich; Paulo Evangelista-Silva; Rodrigo Pereira Prates; Andressa Harumi Torelli Hijo; Tatienne Neder Figueira-Costa; Marie Schaeffer; Francemilson Goulart-Silva; Rodrigo Antonio Peliciari-Garcia; Paula Bargi-Souza

doi:10.1016/j.genrep.2023.101756

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Which housekeeping gene is the best choice for RT-qPCR analysis in mice fed with a high-fat diet? Studies in the liver, kidney, pancreas, and intestines

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Which housekeeping gene is the best choice for RT-qPCR analysis in mice fed with a high-fat diet? Studies in the liver, kidney, pancreas, and intestines

Ayla Secio-Silva, Felipe Emrich, Paulo Evangelista-Silva, Rodrigo Pereira Prates, Andressa Harumi Torelli Hijo, Tatienne Neder Figueira-Costa, Marie Schaeffer, Francemilson Goulart-Silva, Rodrigo Antonio Peliciari-Garcia and Paula Bargi-Souza

Gene Reports, Vol.31

06/2023

DOI: https://doi.org/10.1016/j.genrep.2023.101756

Abstract

gene expression;internal control;Rt-qPCR;obesity;metabolic syndrome;high fat diet

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Title: Which housekeeping gene is the best choice for RT-qPCR analysis in mice fed with a high-fat diet? Studies in the liver, kidney, pancreas, and intestines
Creators - without role: Ayla Secio-Silva - Universidade Federal de Minas Gerais
Felipe Emrich - Universidade Federal de Minas Gerais
Paulo Evangelista-Silva - Universidade de São Paulo
Rodrigo Pereira Prates - Universidade de São Paulo
Andressa Harumi Torelli Hijo - Universidade de São Paulo
Tatienne Neder Figueira-Costa - Universidade de São Paulo
Marie Schaeffer - Université de Montpellier, Institut de Génomique Fonctionnelle - IGF
Francemilson Goulart-Silva - Universidade de São Paulo
Rodrigo Antonio Peliciari-Garcia - Universidade Federal de São Paulo
Paula Bargi-Souza - Universidade Federal de Minas Gerais
Publication Details: Gene Reports, Vol.31
Grant note: Sao Paulo Research Foundation (FAPESP) [FG-S: 2014/12871-9 and PB-S: 2016/24941-7]Brazilian National Council for Scientific and Technological Development (CNPq) [PB-S: 403972/2021–3]Fundação de Amparo à Pesquisa do Estado de Minas Gerais [PB-S: APQ-00013-22]
Identifiers: 9941519409311
Academic Unit: Institut de Génomique Fonctionnelle - IGF; Centre de Biologie Structurale - CBS
Language: English
Resource Type: Journal article
Local Fields: hal-04707766