Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation

Mariana Pavon-Eternod; Alexandre David; Kimberly Dittmar; Peter Berglund; Tao Pan; Jack R. Bennink; Jonathan W. Yewdell

doi:10.1093/nar/gks986

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Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation

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Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation

Mariana Pavon-Eternod, Alexandre David, Kimberly Dittmar, Peter Berglund, Tao Pan, Jack R. Bennink et Jonathan W. Yewdell

Nucleic acids research, Vol.41(3), pp.1914-1921

01/02/2013

DOI: https://doi.org/10.1093/nar/gks986

PMID: 23254333

Résumé

RNA

Transfer RNAs (tRNAs) are central to protein synthesis and impact translational speed and fidelity by their abundance. Here we examine the extent to which viruses manipulate tRNA populations to favor translation of their own genes. We study two very different viruses: influenza A virus (IAV), a medium-sized (13 kB genome) RNA virus; and vaccinia virus (VV), a large (200 kB genome) DNA virus. We show that the total cellular tRNA population remains unchanged following viral infection, whereas the polysome-associated tRNA population changes dramatically in a virus-specific manner. The changes in polysome-associated tRNA levels reflect the codon usage of viral genes, suggesting the existence of local tRNA pools optimized for viral translation.

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Titre: Vaccinia and influenza A viruses select rather than adjust tRNAs to optimize translation
Créateurs - sans rôle: Mariana Pavon-Eternod - University of Chicago
Alexandre David - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 and Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
Kimberly Dittmar - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 and Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
Peter Berglund - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 and Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
Tao Pan - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 and Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
Jack R. Bennink - Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 and Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
Jonathan W. Yewdell - University of Chicago
Détails de publication: Nucleic acids research, Vol.41(3), pp.1914-1921
Éditeur: Oxford University Press
Nombre de pages: 8
Identifiants: 99140062109311
Unité académique: Institut de Recherche en Cancérologie de Montpellier - IRCM
Langue: English
Type de ressource: Journal article