Résumé
Studies in diploid parental species of polyploid plants are important to understand their contributions to the formation of plant and species evolution.
Coffea
eugenioides
is a diploid species that is considered to be an ancestor of allopolyploid
Coffea
arabica
together with
Coffea
canephora
. Despite its importance in the evolutionary history of the main economic species of coffee, no study has focused on
C. eugenioides
molecular genetics. RNA-seq creates the possibility to generate reference transcriptomes and identify coding genes and potential candidates related to important agronomic traits. Therefore, the main objectives were to obtain a global overview of transcriptionally active genes in this species using next-generation sequencing and to analyze specific genes that were highly expressed in leaves and fruits with potential exploratory characteristics for breeding and understanding the evolutionary biology of coffee. A de novo assembly generated 36,935 contigs that were annotated using eight databases. We observed a total of ~5000 differentially expressed genes between leaves and fruits. Several genes exclusively expressed in fruits did not exhibit similarities with sequences in any database. We selected ten differentially expressed unigenes in leaves and fruits to evaluate transcriptional profiles using qPCR. Our study provides the first gene catalog for
C. eugenioides
and enhances the knowledge concerning the mechanisms involved in the
C. arabica
homeologous. Furthermore, this work will open new avenues for studies into specific genes and pathways in this species, especially related to fruit, and our data have potential value in assisted breeding applications.