Abstract
The polyomavirus (PyV) genome is not expressed in undifferentiated embryonal carcinoma (EC) cells such as PCC4 or F9 EC cells. All the viral mutants that have been selected for their expression in these cells harbor mutations or rearrangements within a region which is important for early and late transcription (as transcriptional enhancer) as well as for viral DNA replication. We have studied the role of the different parts of this enhancer on the transcription driven by early PyV promoter. Two series of constructions containing progressive deletions starting from the ends of the region and one series containing linker-scanning mutations were tested in a luciferase assay in three cell lines: 3T3, F9, or PCC4. The results revealed that some regions that have not yet been shown to bind transcription factors are nevertheless important for transcriptional activity. Two subregions between nucleotides 5179 and 5187 and between 5220 and 5227 were found to be inhibitory for the activity of the enhancer in EC cells. The PEA1/AP1 binding site was also unexpectedly shown to be important in F9 EC cells.