Topologically Fixed SecG Is Fully Functional

Eli O. van der Sluis; Erhard van der Vries; Greetje Berrelkamp; Nico Nouwen; Arnold J. M. Driessen

doi:10.1128/JB.188.3.1188-1190.2006

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Topologically Fixed SecG Is Fully Functional

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Topologically Fixed SecG Is Fully Functional

Eli O. van der Sluis, Erhard van der Vries, Greetje Berrelkamp, Nico Nouwen et Arnold J. M. Driessen

Journal of bacteriology, Vol.188(3), pp.1188-1190

01/02/2006

DOI: https://doi.org/10.1128/JB.188.3.1188-1190.2006

PMID: 16428426

Résumé

Microbial Cell Biology

It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

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Détails

Titre: Topologically Fixed SecG Is Fully Functional
Créateurs - sans rôle: Eli O. van der Sluis - Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands
Erhard van der Vries - Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands
Greetje Berrelkamp - Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands
Nico Nouwen - Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands
Arnold J. M. Driessen - Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands
Détails de publication: Journal of bacteriology, Vol.188(3), pp.1188-1190
Éditeur: American Society for Microbiology
Nombre de pages: 3
Identifiants: 99157241109311
Unité académique: Plant Health Institute of Montpellier - PHIM
Langue: English
Type de ressource: Journal article