Abstract
Bioanodes for acetate oxidation were formed with pure cultures of Geobacter sulfurreducens under constant polarization potential. With the original commercial strain, the bioanodes formed at + 0.2 V/SCE exhibited open circuit potential (OCP) of 0.0 V/SCE, while the bioanodes formed at − 0.2 V/SCE had OCP around − 0.52 V/SCE. In contrast, the bioanodes formed with bacterial cells collected from a previous current-producing bioanode exhibited OCP of − 0.52 V/SCE whatever the polarization potential used to form them (+ 0.2 V/SCE or − 0.2 V/SCE). The "electrochemically-adapted strain" kept its electrochemical characteristics after successive cultures in solution. High steady-state currents were reached (16-19 A m- 2) in all cases without any dependence on strain adaptation or applied potential.