The Sulfate Transporter SST1 Is Crucial for Symbiotic Nitrogen Fixation in Lotus japonicus Root Nodules

Lene Krusell; Katja Krause; Thomas Ott; Guilhem Desbrosses; Ute Krämer; Shusei Sato; Yasukazu Nakamura; Satoshi Tabata; Euan K. James; Niels Sandal; Jens Stougaard; Masayoshi Kawaguchi; Ai Miyamoto; Norio Suganuma; Michael K. Udvardi

doi:10.1105/tpc.104.030106

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The Sulfate Transporter SST1 Is Crucial for Symbiotic Nitrogen Fixation in Lotus japonicus Root Nodules

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The Sulfate Transporter SST1 Is Crucial for Symbiotic Nitrogen Fixation in Lotus japonicus Root Nodules

Lene Krusell, Katja Krause, Thomas Ott, Guilhem Desbrosses, Ute Krämer, Shusei Sato, Yasukazu Nakamura, Satoshi Tabata, Euan K. James, Niels Sandal, …

The Plant cell, Vol.17(5), pp.1625-1636

01/05/2005

DOI: https://doi.org/10.1105/tpc.104.030106

PMID: 15805486

Résumé

Symbiotic nitrogen fixation (SNF) by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. Little is known at the molecular level about plant transporters that mediate such exchanges. Several mutants of the model legume Lotus japonicus have been identified that develop nodules with metabolic defects that cannot fix nitrogen efficiently and exhibit retarded growth under symbiotic conditions. Map-based cloning of defective genes in two such mutants, sst1-1 and sst1-2 (for symbiotic sulfate transporter), revealed two alleles of the same gene. The gene is expressed in a nodule-specific manner and encodes a protein homologous with eukaryotic sulfate transporters. Full-length cDNA of the gene complemented a yeast mutant defective in sulfate transport. Hence, the gene was named Sst1. The sst1-1 and sst1-2 mutants exhibited normal growth and development under nonsymbiotic growth conditions, a result consistent with the nodule-specific expression of Sst1. Data from a previous proteomic study indicate that SST1 is located on the symbiosome membrane in Lotus nodules. Together, these results suggest that SST1 transports sulfate from the plant cell cytoplasm to the intracellular rhizobia, where the nutrient is essential for protein and cofactor synthesis, including nitrogenase biosynthesis. This work shows the importance of plant sulfate transport in SNF and the specialization of a eukaryotic transporter gene for this purpose.

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Titre: The Sulfate Transporter SST1 Is Crucial for Symbiotic Nitrogen Fixation in Lotus japonicus Root Nodules
Créateurs - sans rôle: Lene Krusell - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Katja Krause - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Thomas Ott - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Guilhem Desbrosses - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Ute Krämer - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Shusei Sato - Aichi University of Education
Yasukazu Nakamura - Aichi University of Education
Satoshi Tabata - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Euan K. James - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Niels Sandal - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Jens Stougaard - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Masayoshi Kawaguchi - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Ai Miyamoto - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Norio Suganuma - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Michael K. Udvardi - Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark Department of Biological Sciences, University of Tokyo, Hongo, Tokyo 113-0033, Japan Department of Life Science, Aichi University of Education, Kariya, Aichi 448-8542, Japan
Détails de publication: The Plant cell, Vol.17(5), pp.1625-1636
Éditeur: American Society of Plant Biologists
Nombre de pages: 12
Identifiants: 99141232809311
Unité académique: Institut des Sciences des Plantes de Montpellier - IPSiM
Langue: English
Type de ressource: Journal article