Résumé
Recent works indicate that, at specific loci, interactions of chromatin with membrane-less organelles self-assembled through mechanisms of phase separation, like nuclear bodies, are crucial to regulate genome functions, and in particular transcription. Here we describe the protocol of the High-salt Recovered Sequence sequencing method whose principle relies on high-throughput sequencing of genomic DNA trapped into large RNP complexes that are made insoluble by high-salt treatments.