Abstract
Step-by-step chemical peptide synthesis is a largely resolved operation. On the other hand, the coupling of fragments, a key step in the convergent strategy, industrially the most profitable, currently presents great difficulties (see below) when we want to go through chemical activation. In this field, it is interesting to use enzymatic catalysis as an alternative, which therefore appears as a complement to chemical methods. Another niche for the specific use of enzymes in reverse mode is in the amidation and/or C-terminal labeling of peptides prepared by recombinant DNA technology.