Résumé
In cardiac ventricular myocytes, sarcoplasmic reticulum (SR) Ca
2+ load is a key determinant of SR Ca
2+ release. This release normally occurs predominantly from SR junctions at sarcolemmal invaginations (t-tubules), ensuring synchronous SR Ca
2+ release throughout the cell. However under conditions of Ca
2+ overload, spontaneous SR Ca
2+ release and propagating Ca
2+ waves can occur, which are pro-arrhythmic. We used detubulated rat ventricular myocytes to determine the dependence of Ca
2+ wave propagation on SR Ca
2+ load, and the role of t-tubules in SR Ca
2+ uptake and spontaneous release. After SR Ca
2+ depletion, recovery of Ca
2+ transient amplitude (and SR Ca
2+ load) was slower in detubulated than control myocytes (half-maximal recovery: 9.9
±
1.4 vs. 5.5
±
0.7 beats). In detubulated myocytes the extent and velocity of Ca
2+ propagation from the cell periphery increased with each beat and depended steeply on SR Ca
2+ load. Isoproterenol (ISO) accelerated recovery, increased maximal propagation velocity and reduced the threshold SR Ca
2+ load for propagation. Ca
2+ spark frequency was uniform across control cell width and was similar at the periphery of detubulated cells. However, internal Ca
2+ spark frequency in detubulated cells was 75% lower (despite comparable local SR Ca
2+ load); this transverse spark frequency profile was similar to that in atrial myocytes. We conclude that: (1) t-tubule Ca
2+ fluxes normally control SR Ca
2+ refilling; (2) Ca
2+ wave propagation depends steeply on SR Ca
2+ content (3) SR-t-tubule junctions are important in initiating SR Ca
2+ release and (4) ISO enhances propagation of SR Ca release, but not the initiation of SR Ca release events (for given SR Ca
2+ loads).