Single cell analysis defines the divergence between the innate lymphoid cell and lymphoid tissue inducer lineages

Isabel E. Ishizuka; Sylvestre Chea; Herman Gudjonson; Michael G. Constantinides; Aaron R. Dinner; Albert Bendelac; Rachel Golub

doi:10.1038/ni.3344

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Single cell analysis defines the divergence between the innate lymphoid cell and lymphoid tissue inducer lineages

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Single cell analysis defines the divergence between the innate lymphoid cell and lymphoid tissue inducer lineages

Isabel E. Ishizuka, Sylvestre Chea, Herman Gudjonson, Michael G. Constantinides, Aaron R. Dinner, Albert Bendelac et Rachel Golub

Nature immunology, Vol.17(3), pp.269-276

18/01/2016

DOI: https://doi.org/10.1038/ni.3344

PMID: 26779601

Résumé

The precise lineage relationship between innate lymphoid cells (ILC) and lymphoid tissue inducer (LTi) cells is poorly understood. Using single-cell multiplex transcriptional analysis of 100 lymphoid genes and single-cell cultures of fetal liver precursor cells, we identified the common proximal precursor to these lineages and showed that its bifurcation was marked by the differential induction of the transcription factors PLZF and TCF1. Acquisition of individual ILC subset-specific effector programs was initiated later, at the common ILC precursor stage, by transient expression of mixed ILC1, ILC2 and ILC3 transcriptional patterns whereas, in contrast, LTi cell development did not go through multilineage priming. These findings provide insights into divergent mechanisms of ILC and LTi cell lineage differentiation and establish a high-resolution blueprint of their development.

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Titre: Single cell analysis defines the divergence between the innate lymphoid cell and lymphoid tissue inducer lineages
Créateurs - sans rôle: Isabel E. Ishizuka - Committee on Immunology, University of Chicago, Chicago IL, 60637, USA Department of Pathology, University of Chicago, Chicago IL, 60637, USA Institut Pasteur, Immunology Department, Lymphopoiesis Unit, Inserm U668, University Paris Diderot, Paris, France Institute of Biophysical Dynamics, University of Chicago, Chicago IL, 60637, USA Department of Chemistry, University of Chicago, Chicago IL, 60637, USA
Sylvestre Chea - Committee on Immunology, University of Chicago, Chicago IL, 60637, USA Department of Pathology, University of Chicago, Chicago IL, 60637, USA Institut Pasteur, Immunology Department, Lymphopoiesis Unit, Inserm U668, University Paris Diderot, Paris, France Institute of Biophysical Dynamics, University of Chicago, Chicago IL, 60637, USA Department of Chemistry, University of Chicago, Chicago IL, 60637, USA
Herman Gudjonson - Committee on Immunology, University of Chicago, Chicago IL, 60637, USA Department of Pathology, University of Chicago, Chicago IL, 60637, USA Institut Pasteur, Immunology Department, Lymphopoiesis Unit, Inserm U668, University Paris Diderot, Paris, France Institute of Biophysical Dynamics, University of Chicago, Chicago IL, 60637, USA Department of Chemistry, University of Chicago, Chicago IL, 60637, USA
Michael G. Constantinides - Committee on Immunology, University of Chicago, Chicago IL, 60637, USA Department of Pathology, University of Chicago, Chicago IL, 60637, USA Institut Pasteur, Immunology Department, Lymphopoiesis Unit, Inserm U668, University Paris Diderot, Paris, France Institute of Biophysical Dynamics, University of Chicago, Chicago IL, 60637, USA Department of Chemistry, University of Chicago, Chicago IL, 60637, USA
Aaron R. Dinner - Committee on Immunology, University of Chicago, Chicago IL, 60637, USA Department of Pathology, University of Chicago, Chicago IL, 60637, USA Institut Pasteur, Immunology Department, Lymphopoiesis Unit, Inserm U668, University Paris Diderot, Paris, France Institute of Biophysical Dynamics, University of Chicago, Chicago IL, 60637, USA Department of Chemistry, University of Chicago, Chicago IL, 60637, USA
Albert Bendelac - Committee on Immunology, University of Chicago, Chicago IL, 60637, USA Department of Pathology, University of Chicago, Chicago IL, 60637, USA Institut Pasteur, Immunology Department, Lymphopoiesis Unit, Inserm U668, University Paris Diderot, Paris, France Institute of Biophysical Dynamics, University of Chicago, Chicago IL, 60637, USA Department of Chemistry, University of Chicago, Chicago IL, 60637, USA
Rachel Golub - Committee on Immunology, University of Chicago, Chicago IL, 60637, USA Department of Pathology, University of Chicago, Chicago IL, 60637, USA Institut Pasteur, Immunology Department, Lymphopoiesis Unit, Inserm U668, University Paris Diderot, Paris, France Institute of Biophysical Dynamics, University of Chicago, Chicago IL, 60637, USA Department of Chemistry, University of Chicago, Chicago IL, 60637, USA
Détails de publication: Nature immunology, Vol.17(3), pp.269-276
Identifiants: 99155836709311
Unité académique: Cell souches plasticité cellul régénérat tissulaire immuno des maladies inflamm - IRMB
Langue: English
Type de ressource: Journal article