Abstract
Silica-based nanoparticles have been extensively investigated as advanced bioimaging probes and smart nanocarriers for the development of nanomedicine because of their unique properties. The deep understanding of nanoparticle-biological system (nanobiology) interactions is an important determinant for the success of nanomedicine. In this Review, we focus on the assessment of significant nanobio interactions along with the intracellular fates of silica-based nanoparticles by advanced microscopy and link this information to their recent biomedical applications. Initially, the fundamental concepts of the dynamic interactions between nanoparticles and proteins followed by cells and key factors that influence these nanobio interactions are briefly introduced. Furthermore, the basic principles of advanced imaging modalities for the analysis of nanobio interactions in this study are described. Next, the utilization of advanced imaging for the characterization of protein coronas and monitoring the cellular internalization and intracellular trafficking/fate of individual nanoparticles are comprehensively summarized. Finally, recent biomedical applications of nanoparticles in bioimaging and drug delivery are discussed.