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Reduction with tris(2-carboxyethyl)phosphine (TCEP) enables the use of an S-sulphonate protecting group for thiol-mediated bioconjugation.
Journal article   Peer reviewed

Reduction with tris(2-carboxyethyl)phosphine (TCEP) enables the use of an S-sulphonate protecting group for thiol-mediated bioconjugation.

Barbara Maret, Thomas Régnier, Jean-Christophe Rossi, Laurent Garrelly, Laurent Vial and Robert Pascal
RSC Advances, Vol.4(15), pp.7725-728
10/01/2014

Abstract

tris(2-carboxyethyl)phosphine TCEP S-sulphonate thiol-mediated bioconjugation thiol protecting
Herein, we demonstrate the effectiveness of the water-friendly S-sulphonate group as an alternative to traditional thiol protecting groups for subsequent deprotection-bioconjugation reactions, under conditions that are compatible with the use of biochemical samples.
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