RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

Olle Terenius; Alexie Papanicolaou; Jennie S. Garbutt; Ioannis Eleftherianos; Hanneke Huvenne; Sriramana Kanginakudru; Merete Albrechtsen; Chunju An; Jean-Luc Aymeric; Andrea Barthel; Piotr Bebas; Kavita Bitra; Alejandra Bravo; Francois Chevalieri; Derek P. Collinge; Cristina M. Crava; Ruud A. de Maagd; Bernard Duvic; Martin Erlandson; Ingrid Faye; Gabriella Felfoeldi; Haruhiko Fujiwara; Ryo Futahashi; Archana S. Gandhe; Heather S. Gatehouse; Laurence N. Gatehouse; Jadwiga M. Giebultowicz; Isabel Gómez; Cornelis J. P. Grimmelikhuijzen; Astrid T. Groot; Frank Hauser; David G. Heckel; Dwayne D. Hegedus; Steven Hrycaj; Lihua Huang; J. Joe Hull; Kostas Iatrou; Masatoshi Iga; Michael R. Kanost; Joanna Kotwica; Changyou Li; Jianghong Li; Jisheng Liu; Magnus Lundmark; Shogo Matsumoto; Martina Meyering-Vos; Peter J. Millichap; Antonia Monteiro; Nirotpal Mrinal; Teruyuki Niimi; Daniela Nowara; Atsushi Ohnishi; Vicencio Oostra; Katsuhisa Ozaki; Maria Papakonstantinou; Aleksandar Popadic; Manchikatla V. Rajam; Suzanne Saenko; Robert M. Simpson; Mario Soberon; Michael R. Strand; Shuichiro Tomita; Umut Toprak; Ping Wang; Choon Wei Wee; Steven Whyard; Wenqing Zhang; Javaregowda Nagaraju; Richard H. Ffrench-Constant; Salvador Herrero; Karl Gordon; Luc Swelters; Guy Smagghe

doi:10.1016/j.jinsphys.2010.11.006

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RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

Journal article

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RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

Olle Terenius, Alexie Papanicolaou, Jennie S. Garbutt, Ioannis Eleftherianos, Hanneke Huvenne, Sriramana Kanginakudru, Merete Albrechtsen, Chunju An, Jean-Luc Aymeric, Andrea Barthel, …

Journal of Insect Physiology, Vol.57(2), pp.231 - 245

2011

DOI: https://doi.org/10.1016/j.jinsphys.2010.11.006

Abstract

RNA interference

Delivery methods

Tissue uptake

Gene function

dsRNA properties

DOUBLE-STRANDED-RNA

ARMYWORM SPODOPTERA-FRUGIPERDA

SILKWORM BOMBYX-MORI

SMALL SILENCING RNAS

CAENORHABDITIS-ELEGANS

Lepidoptera

MANDUCA-SEXTA

BACILLUS-THURINGIENSIS

HELICOVERPA-ARMIGERA

IMMUNE-RESPONSES

MESSENGER-RNA

Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. (C) 2010 Elsevier Ltd. All rights reserved.

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Title: RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design
Creators - without role: Olle Terenius - Swedish University of Agricultural Sciences
Alexie Papanicolaou - University of Exeter
Jennie S. Garbutt - University of Bath
Ioannis Eleftherianos - George Washington University
Hanneke Huvenne - Ghent University Hospital
Sriramana Kanginakudru - Centre for DNA Fingerprinting and Diagnostics
Merete Albrechtsen - University of Copenhagen
Chunju An - Kansas State University
Jean-Luc Aymeric - Université de Montpellier
Andrea Barthel - Max Planck Society
Piotr Bebas - University of Warsaw
Kavita Bitra - University of Georgia
Alejandra Bravo - Universidad Nacional Autónoma de México
Francois Chevalieri - Université Montpellier 2
Derek P. Collinge - Australian National University
Cristina M. Crava - Universitat de València
Ruud A. de Maagd - Wageningen University & Research
Bernard Duvic - Université de Montpellier
Martin Erlandson - University of Saskatchewan
Ingrid Faye - Stockholm University
Gabriella Felfoeldi - Eötvös Loránd University
Haruhiko Fujiwara - The University of Tokyo
Ryo Futahashi - National Institute of Advanced Industrial Science and Technology
Archana S. Gandhe - Centre for DNA Fingerprinting and Diagnostics
Heather S. Gatehouse - Plant & Food Research
Laurence N. Gatehouse - Plant & Food Research
Jadwiga M. Giebultowicz - Oregon State University
Isabel Gómez - Universidad Nacional Autónoma de México
Cornelis J. P. Grimmelikhuijzen - University of Copenhagen
Astrid T. Groot - Max Planck Innovation
Frank Hauser - University of Copenhagen
David G. Heckel - Max Planck Innovation
Dwayne D. Hegedus - University of Saskatchewan
Steven Hrycaj - University of Exeter
Lihua Huang - Health Sciences and Nutrition
J. Joe Hull - Agricultural Research Service
Kostas Iatrou - Ghent University Hospital
Masatoshi Iga - Ghent University Hospital
Michael R. Kanost - Kansas State University
Joanna Kotwica - University of Warsaw
Changyou Li - Commonwealth Scientific and Industrial Research Organisation
Jianghong Li
Jisheng Liu - Ghent University Hospital
Magnus Lundmark - University of Copenhagen
Shogo Matsumoto - University of Bath
Martina Meyering-Vos - Centre for DNA Fingerprinting and Diagnostics
Peter J. Millichap - University of Bath
Antonia Monteiro - University of Copenhagen
Nirotpal Mrinal - Centre for DNA Fingerprinting and Diagnostics
Teruyuki Niimi - Kansas State University
Daniela Nowara - University of Copenhagen
Atsushi Ohnishi - University of Bath
Vicencio Oostra - Leiden University
Katsuhisa Ozaki - Australian National University
Maria Papakonstantinou - Ghent University Hospital
Aleksandar Popadic - University of Exeter
Manchikatla V. Rajam - University of Warsaw
Suzanne Saenko - Leiden University
Robert M. Simpson - Plant & Food Research
Mario Soberon - Universidad Nacional Autónoma de México
Michael R. Strand - University of Georgia
Shuichiro Tomita - University of Georgia
Umut Toprak - University of Saskatchewan
Ping Wang - Commonwealth Scientific and Industrial Research Organisation
Choon Wei Wee - Australian National University
Steven Whyard
Wenqing Zhang - Wageningen University & Research
Javaregowda Nagaraju - Centre for DNA Fingerprinting and Diagnostics
Richard H. Ffrench-Constant - University of Exeter
Salvador Herrero - Universitat de València
Karl Gordon - Health Sciences and Nutrition
Luc Swelters
Guy Smagghe - Ghent University Hospital
Publication Details: Journal of Insect Physiology, Vol.57(2), pp.231 - 245
Identifiers: 9933758409311
Academic Unit: Université de Montpellier
Language: English
Resource Type: Journal article
Local Fields: hal-02652706