Quantitative analysis of chromatin compaction in living cells using FLIM–FRET

Journal article

Open access

Peer reviewed

David Llères, John James, Sam Swift, David G Norman and Angus I Lamond

Journal of Cell Biology, Vol.187(4), pp.481 - 496

15/12/2009

Abstract

2-DG

2-deoxyglucose

5-FU

5-fluorouracil

CENP-C

centromere protein C

FLIM

fluorescence lifetime imaging microscopy

FRET

Förster resonance energy transfer

H3K9triMe

histone H3 lysine trimethylation

MNase

micrococcal nuclease

MPLSM

multiphoton laser-scanning microscopy

PB

physiological buffer

ROI

region of interest

TCSPC

timecorrelated single-photon counting

TEM

transmission EM

TSA

trichostatin A

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Title: Quantitative analysis of chromatin compaction in living cells using FLIM–FRET
Creators - without role: David Llères - Université de Montpellier, Institut de Génétique Moléculaire de Montpellier - IGMM
John James - Cancer Research UK
Sam Swift - University of Dundee
David G Norman - University of Dundee
Angus I Lamond - University of Dundee
Publication Details: Journal of Cell Biology, Vol.187(4), pp.481 - 496
Identifiers: 9946852109311
Academic Unit: Institut de Génétique Moléculaire de Montpellier - IGMM
Language: English
Resource Type: Journal article
Local Fields: hal-03027048