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Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model
Article de revue   Open Access   Avec comité de lecture

Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model

Tommy Chastel, Serena Filiberti, Stefania Mitola, Roberto Ronca, Andrei Turtoi et Michela Corsini
STAR Protocols, Vol.6(1)
03/2025
DOI: https://doi.org/10.1016/j.xpro.2025.103663

Résumé

Model organisms Cell biology Cancer Animals Carcinogenesis / pathology Chick Embryo Chorioallantoic Membrane / blood supply Chorioallantoic Membrane / pathology Neovascularization, Pathologic / pathology Neovascularization, Physiologic
The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model. We describe steps for initial general preparation and for the angiogenic and tumorigenic assays in two distinct sections.

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