Résumé
We demonstrate the use of a chromosomal walk (or “tiling path”)
printed as DNA microarrays for mapping protein–DNA interactions across
large regions of contiguous genomic DNA in Drosophila melanogaster.
Microarrays were constructed with genomic DNA fragments 430–920 bp in
length, covering 2.9 million base pairs of the
Adh–cactus region of chromosome 2 and 85,000 base
pairs of the 82F region of chromosome 3. We performed DNA
localization mapping for the heterochromatin protein HP1 and for the
sequence-specific GAGA transcription factor, producing a comprehensive,
high-resolution map of in vivo protein–DNA interactions
throughout these regions of the Drosophila genome.