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Production and purification of fumonisins from a stirred jar fermenter
Article de revue scientifique   Avec comité de lecture

Production and purification of fumonisins from a stirred jar fermenter

J. David Miller, Marc E. Savard et Sylvie Rapior
Natural Toxins, Vol.2(6), p.354-359
29/07/1994
PMID: 7704448

Résumé

Carcinogens, Environmental Chromatography, High Pressure Liquid Sucrose Chromatography, Ion Exchange Chromatography, Thin Layer Culture Media Fermentation Fructose Fumonisins Fusarium Glucose Mycotoxins Oxygen Consumption
The production, isolation and purification of fumonisins from 10 litre liquid cultures are described. Measurements of sucrose, fructose and glucose consumption, oxygen demand, dry weight increase, CO2, and fumonisin production were taken every 48 hours. The specific productivity of fumonisins was found to be similar to that reported for corn cultures but purification was much simpler, yielding an 89% recovery. The method developed for the purification of fumonisins from liquid culture was also applied to a corn culture, resulting in a 70.1% recovery.

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