Polymorphic chromosomal inversions in Anopheles moucheti, a major malaria vector in Central Africa

Maria V. Sharakhova; Christophe Antonio-Nkondjio; Ai Xia; Cyrille Ndo; Parfait Awono-Ambene; Frederic Simard; Igor V. Sharakhov

doi:10.1111/mve.12037

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Polymorphic chromosomal inversions in Anopheles moucheti, a major malaria vector in Central Africa

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Polymorphic chromosomal inversions in Anopheles moucheti, a major malaria vector in Central Africa

Maria V. Sharakhova, Christophe Antonio-Nkondjio, Ai Xia, Cyrille Ndo, Parfait Awono-Ambene, Frederic Simard et Igor V. Sharakhov

Medical and veterinary entomology, Vol.28(3), pp.337-340

09/2014

DOI: https://doi.org/10.1111/mve.12037

PMID: 24192050

Résumé

Anopheles moucheti Evans (Diptera, Culicidae) is a major vector of malaria in forested areas of Central Africa. However, few genetic tools are available for this species. The present study represents the first attempt to characterize chromosomes in An. moucheti females collected in Cameroon. Ovarian nurse cells contained polytene chromosomes, which were suitable for standard cytogenetic applications. The presence of three polymorphic chromosomal inversions in An. moucheti was revealed. Two of these inversions were located on 2R chromosomal arm. The homology between 2R chromosomal arms of An. moucheti and An. gambiae Giles was established by fluorescent in situ hybridization of six An. gambiae genic sequences. Mapping of the probes on chromosomes of An. moucheti detected substantial gene order reshuffling between the two species. The presence of polytene chromosomes and polymorphic inversions in An. moucheti provides a new tool for population genetic, taxonomic, and ecological studies of this neglected malaria vector.

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Titre: Polymorphic chromosomal inversions in Anopheles moucheti, a major malaria vector in Central Africa
Créateurs - sans rôle: Maria V. Sharakhova - Department of Entomology, 203 Fralin Life Science Institute, MC 0346, Virginia Tech, Blacksburg, VA 24061, USA Malaria Research Laboratory, OCEAC, PO Box 288, Yaoundé, Cameroon MIVEGEC (UMR IRD224-CNRS5290-UM1-UM2), Institut de Recherche pour le Développement (IRD), BP 64501, 34394, Montpellier, Cedex 5, France Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon
Christophe Antonio-Nkondjio - University of Douala
Ai Xia - Department of Entomology, 203 Fralin Life Science Institute, MC 0346, Virginia Tech, Blacksburg, VA 24061, USA Malaria Research Laboratory, OCEAC, PO Box 288, Yaoundé, Cameroon MIVEGEC (UMR IRD224-CNRS5290-UM1-UM2), Institut de Recherche pour le Développement (IRD), BP 64501, 34394, Montpellier, Cedex 5, France Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon
Cyrille Ndo - Department of Entomology, 203 Fralin Life Science Institute, MC 0346, Virginia Tech, Blacksburg, VA 24061, USA Malaria Research Laboratory, OCEAC, PO Box 288, Yaoundé, Cameroon MIVEGEC (UMR IRD224-CNRS5290-UM1-UM2), Institut de Recherche pour le Développement (IRD), BP 64501, 34394, Montpellier, Cedex 5, France Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon
Parfait Awono-Ambene - University of Douala
Frederic Simard - Department of Entomology, 203 Fralin Life Science Institute, MC 0346, Virginia Tech, Blacksburg, VA 24061, USA Malaria Research Laboratory, OCEAC, PO Box 288, Yaoundé, Cameroon MIVEGEC (UMR IRD224-CNRS5290-UM1-UM2), Institut de Recherche pour le Développement (IRD), BP 64501, 34394, Montpellier, Cedex 5, France Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon
Igor V. Sharakhov - Department of Entomology, 203 Fralin Life Science Institute, MC 0346, Virginia Tech, Blacksburg, VA 24061, USA Malaria Research Laboratory, OCEAC, PO Box 288, Yaoundé, Cameroon MIVEGEC (UMR IRD224-CNRS5290-UM1-UM2), Institut de Recherche pour le Développement (IRD), BP 64501, 34394, Montpellier, Cedex 5, France Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Douala, Cameroon
Détails de publication: Medical and veterinary entomology, Vol.28(3), pp.337-340
Nombre de pages: 4
Identifiants: 99147848209311
Unité académique: Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle - MIVEGEC
Langue: English
Type de ressource: Journal article