Platelets Disseminate Extracellular Vesicles in Lymph in Rheumatoid Arthritis

Nicolas Tessandier; Imene Melki; Nathalie Cloutier; Isabelle Allaeys; Adam Miszta; Sisareuth Tan; Andreea Milasan; Sara Michel; Abderrahim Benmoussa; Tania Lévesque; Francine Côté; Steven E McKenzie; Caroline Gilbert; Patrick Provost; Alain R Brisson; Alisa S Wolberg; Paul R Fortin; Catherine Martel; Éric Boilard

doi:10.1161/ATVBAHA.119.313698

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Platelets Disseminate Extracellular Vesicles in Lymph in Rheumatoid Arthritis

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Platelets Disseminate Extracellular Vesicles in Lymph in Rheumatoid Arthritis

Nicolas Tessandier, Imene Melki, Nathalie Cloutier, Isabelle Allaeys, Adam Miszta, Sisareuth Tan, Andreea Milasan, Sara Michel, Abderrahim Benmoussa, Tania Lévesque, …

Arteriosclerosis, thrombosis, and vascular biology, Vol.40(4), pp.929-942

04/2020

DOI: https://doi.org/10.1161/ATVBAHA.119.313698

PMID: 32102567

Résumé

Animals

Arthritis, Rheumatoid - physiopathology

Blood Platelets - metabolism

Blood Platelets - physiology

Capillary Permeability

Disease Models, Animal

Extracellular Vesicles - physiology

Lymph - physiology

Mice, Inbred C57BL

Serotonin - metabolism

The lymphatic system is a circulatory system that unidirectionally drains the interstitial tissue fluid back to blood circulation. Although lymph is utilized by leukocytes for immune surveillance, it remains inaccessible to platelets and erythrocytes. Activated cells release submicron extracellular vesicles (EV) that transport molecules from the donor cell. In rheumatoid arthritis, EV accumulate in the joint where they can interact with numerous cellular lineages. However, whether EV can exit the inflamed tissue to recirculate is unknown. Here, we investigated whether vascular leakage that occurs during inflammation could favor EV access to the lymphatic system. Approach and Results: Using an in vivo model of autoimmune inflammatory arthritis, we show that there is an influx of platelet EV, but not EV from erythrocytes or leukocytes, in joint-draining lymph. In contrast to blood platelet EV, lymph platelet EV lacked mitochondrial organelles and failed to promote coagulation. Platelet EV influx in lymph was consistent with joint vascular leakage and implicated the fibrinogen receptor α2bβ and platelet-derived serotonin. These findings show that platelets can disseminate their EV in fluid that is inaccessible to platelets and beyond the joint in this disease.

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Titre: Platelets Disseminate Extracellular Vesicles in Lymph in Rheumatoid Arthritis
Créateurs - sans rôle: Nicolas Tessandier - Département de microbiologie-infectiologie et d'immunologie, Université Laval, QC, Canada (N.T., I.M., N.C., I.A., S.M., T.L., C.G., P.P., P.R.F., E.B.)
Imene Melki - Département de microbiologie-infectiologie et d'immunologie, Université Laval, QC, Canada (N.T., I.M., N.C., I.A., S.M., T.L., C.G., P.P., P.R.F., E.B.)
Nathalie Cloutier - Département de microbiologie-infectiologie et d'immunologie, Université Laval, QC, Canada (N.T., I.M., N.C., I.A., S.M., T.L., C.G., P.P., P.R.F., E.B.)
Isabelle Allaeys - Département de microbiologie-infectiologie et d'immunologie, Université Laval, QC, Canada (N.T., I.M., N.C., I.A., S.M., T.L., C.G., P.P., P.R.F., E.B.)
Adam Miszta - Montreal Heart Institute, Quebec, Canada (A.M., C.M.)
Sisareuth Tan - Extracellular Vesicles and Membrane Repair, UMR-5248-CBMN CNRS-University of Bordeaux-IPB, Allée Geoffroy Saint-Hilaire, Pessac, France (S.T., A.R.B.)
Andreea Milasan - Department of Medicine, Faculty of Medicine (A.M., C.M.), Université de Montréal, Quebec, Canada
Sara Michel - Département de microbiologie-infectiologie et d'immunologie, Université Laval, QC, Canada (N.T., I.M., N.C., I.A., S.M., T.L., C.G., P.P., P.R.F., E.B.)
Abderrahim Benmoussa - Department of Nutrition, CHU Sainte-Justine (A.B.), Université de Montréal, Quebec, Canada
Tania Lévesque - Département de microbiologie-infectiologie et d'immunologie, Université Laval, QC, Canada (N.T., I.M., N.C., I.A., S.M., T.L., C.G., P.P., P.R.F., E.B.)
Francine Côté - Institut Imagine, Inserm U1163, Laboratoire Olivier Hermine, Paris, France (F.C.)
Steven E McKenzie - Cardeza Foundation for Hematological Research, Thomas Jefferson University, Philadelphia, PA (S.E.M.)
Caroline Gilbert - Département de microbiologie-infectiologie et d'immunologie, Université Laval, QC, Canada (N.T., I.M., N.C., I.A., S.M., T.L., C.G., P.P., P.R.F., E.B.)
Patrick Provost - Département de microbiologie-infectiologie et d'immunologie, Université Laval, QC, Canada (N.T., I.M., N.C., I.A., S.M., T.L., C.G., P.P., P.R.F., E.B.)
Alain R Brisson - Extracellular Vesicles and Membrane Repair, UMR-5248-CBMN CNRS-University of Bordeaux-IPB, Allée Geoffroy Saint-Hilaire, Pessac, France (S.T., A.R.B.)
Alisa S Wolberg - Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill (A.M., A.S.W.)
Paul R Fortin - Axe maladies infectieuses et inflammatoires, Centre de recherche du CHU de Québec-Université Laval, Québec, Canada (P.R.F., E.B.)
Catherine Martel - Montreal Heart Institute, Quebec, Canada (A.M., C.M.)
Éric Boilard - Centre de recherche du CHU de Québec-Université Laval
Détails de publication: Arteriosclerosis, thrombosis, and vascular biology, Vol.40(4), pp.929-942
Note de subvention: CIHR R01 HL126974 / NHLBI NIH HHS
Identifiants: 99147800309311
Unité académique: Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle - MIVEGEC
Langue: English
Type de ressource: Journal article