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Phylogenetic taxonomy of Leishmania (Viannia) braziliensis based on isoenzymatic study of 137 isolates
Article de revue scientifique   Avec comité de lecture

Phylogenetic taxonomy of Leishmania (Viannia) braziliensis based on isoenzymatic study of 137 isolates

N Chouicha, G Lanotte, F Pratlong, C A Cuba Cuba, I D Velez et J P Dedet
Parasitology, Vol.115 ( Pt 4)(4), p.343-348
01/10/1997
PMID: 9364560

Résumé

Americas - epidemiology Animals Classification Evolution, Molecular Humans Isoenzymes Leishmania braziliensis - classification Leishmania braziliensis - enzymology Leishmaniasis, Cutaneous - epidemiology Leishmaniasis, Cutaneous - parasitology Phylogeny Polymorphism, Genetic Psychodidae - parasitology
Biochemical characterization of 137 Leishmania braziliensis isolates from South and Central America, and from selected endemic foci in Bolivia, Brazil and Colombia, performed by isoenzymatic electrophoresis using 10 enzymatic systems, showed a high enzymatic polymorphism (44 zymodemes obtained) based on the variation of a small number of enzymes. Cladistic analysis showed close links between the zymodemes within the L. braziliensis s.s. cluster. The position of 2 Colombian zymodemes obtained (MON*204 and MON*205) justify the inclusion of L. peruviana within the L. braziliensis cluster.

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