Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens

Pratt Kolia-Diafouka; Sylvain Godreuil; Arnaud Bourdin; Severine Carrer-Kremer; Laurent Kremer; Philippe van de Perre; Edouard Tuaillon

doi:10.3389/fmicb.2018.02224

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Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens

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Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens

Pratt Kolia-Diafouka, Sylvain Godreuil, Arnaud Bourdin, Severine Carrer-Kremer, Laurent Kremer, Philippe van de Perre et Edouard Tuaillon

Frontiers in Microbiology, Vol.9

25/09/2018

DOI: https://doi.org/10.3389/fmicb.2018.02224

Résumé

Background: When available, nucleic acid tests (NATs) offer powerful tools to strengthen the potential of tuberculosis (TB) diagnosis assays. The sensitivity of molecular assays is critical for detection of Mycobacterium tuberculosis (MTB) in paucibacillary sputum.

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Titre: Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens
Créateurs - sans rôle: Pratt Kolia-Diafouka - Université de Montpellier, Pathogenesis and Control of Chronic and Emerging Infections - PCCEI
Sylvain Godreuil - Université de Montpellier
Arnaud Bourdin - Université de Montpellier, Physiologie et Médecine Expérimentale du coeur et des muscles - PhyMedExp
Severine Carrer-Kremer - Université de Montpellier, Pathogenesis and Control of Chronic and Emerging Infections - PCCEI
Laurent Kremer - Université de Montpellier, Institut de Recherche en Infectiologie de Montpellier - IRIM
Philippe van de Perre - Université de Montpellier, Pathogenesis and Control of Chronic and Emerging Infections - PCCEI
Edouard Tuaillon - Hôpital Arnaud de Villeneuve
Détails de publication: Frontiers in Microbiology, Vol.9
Identifiants: 99152143409311
Unité académique: Physiologie et Médecine Expérimentale du coeur et des muscles - PhyMedExp; Pathogenesis and Control of Chronic and Emerging Infections - PCCEI; Institut de Recherche en Infectiologie de Montpellier - IRIM
Langue: English
Type de ressource: Journal article
Champs locaux: hal-01887123