Résumé
•Optimization of somatic embryogenesis protocol revealed the predominant role of balances in growth regulators.•This optimization should simultaneously control callus growth, tissue browning and embryogenic capacity of cells.•An optimized protocol for somatic embryogenesis facilitates the production of transgenic lines.
The challenges for the development of rubber cultivation are so agronomically significant in the face of climate change that it is crucial to introduce biotechnology into the clonal propagation of rubber trees. A protocol was developed on rubber clone PB 260. This protocol was used for two other clones, RRIM 600 and REYAN 88-13, which exhibited highly contrasting response to callus growth and embryogenic capacity. Optimization of somatic embryogenesis for these two clones led us to successful conduct genetic transformation trials. We discussed the in vitro behaviour of the calli considering the origin of the embryogenic callus and the endogenous redox status.