Résumé
Various chimeric oligonucleotides consisting of α- and β-anomeric deoxynucleotide units have been synthesized and their hybridizing abilities to complementary DNA or RNA single strands, and their stability in cell culture medium or cell extracts, were studied. The results provide guide lines for optimization of antisense chimeric oligodeoxynucleotides.Various chimeric oligonucleotides consisting of α- and β-anomeric deoxynucleotide units have been synthesized and their hybridizing abilities to complementary DNA or RNA single strands, and their stability in cell culture medium or cell extracts, were studied. The results provide guide lines for optimization of antisense chimeric oligodeoxynucleotides.