Abstract
level quantitation is widely used as an invariant control for gene expression analysis. Only three vertebrate GAPDH cDNAs have been hitherto isolated: human (1), rat (2) and chick (3). In order to get a probe for RNAse-mapping experiments on hamster (Cricetulus griseus) mRNAs, we constructed a cDNA library in pBluescript KSII (Stratagene) from CCL39 cell line (adult lung fibroblastic cells). A full-length cDNA encoding GAPDH was then isolated. DNA sequence was determined on both strands and processed using BISANCE package (CITI2, Paris). The sequence spans 1266 nucleotides, and exhibits a high degree of identity to each of the previously known vertebrates sequences. Northern blot analysis (not shown) revealed the presence of a single mRNA species expressed from hamster GAPDH multigenic family (4).