Multiple integrations of a sense transgene, including a tandem inverted repeat confer stable RNA-silencing mediated virus resistance under different abiotic and biotic conditions

Despoina Beris; Aliki Tzima; Fani Gousi; Aggeliki Rampou; Venetia Psarra; Ioannis Theologidis; Nikon Vassilakos

doi:10.1007/s11248-023-00333-1

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Multiple integrations of a sense transgene, including a tandem inverted repeat confer stable RNA-silencing mediated virus resistance under different abiotic and biotic conditions

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Multiple integrations of a sense transgene, including a tandem inverted repeat confer stable RNA-silencing mediated virus resistance under different abiotic and biotic conditions

Despoina Beris, Aliki Tzima, Fani Gousi, Aggeliki Rampou, Venetia Psarra, Ioannis Theologidis et Nikon Vassilakos

Transgenic research, Vol.32(1-2), pp.53-66

04/2023

DOI: https://doi.org/10.1007/s11248-023-00333-1

PMID: 36633706

Résumé

Nicotiana - genetics

Nicotiana - metabolism

Plant Diseases - genetics

Plant Viruses - genetics

Plants, Genetically Modified - metabolism

RNA Interference

RNA, Small Interfering - genetics

Transgenes

In a previous study, tobacco plants, transformed with a sense construct of the 57K domain of the replicase gene of tobacco rattle virus (TRV), provided resistance against genetically distant isolates of the virus. In this work, 57K-specific siRNAs were detected with RT-qPCR solely in the resistant line verifying the RNA-silencing base of the resistance. The integration sites of the transgene into the plant genome were identified with inverse-PCR. Moreover, the resistance against TRV was practically unaffected by low temperature conditions and the presence of heterologous viruses. The mechanism of the resistance was further examined by a gene expression analysis that showed increased transcript levels of genes with a key-role in the RNA silencing pathway and the basal antiviral defence. This work provides a comprehensive characterization of the robust virus resistance obtained by a sense transgene and underlines the usefulness of transgenic plants obtained by such a strategy.

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Titre: Multiple integrations of a sense transgene, including a tandem inverted repeat confer stable RNA-silencing mediated virus resistance under different abiotic and biotic conditions
Créateurs - sans rôle: Despoina Beris - Laboratory of Virology, Scientific Directorate of Phytopathology, Benaki Phytopathological Institute, 8 Stefanou Delta Street, 14561, Athens, Greece. d.mperi@bpi.gr
Aliki Tzima - Laboratory of Plant Pathology, Department of Crop Production, School of Agricultural Production Infrastructure and Environment, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece
Fani Gousi - Laboratory of Plant Pathology, Department of Crop Production, School of Agricultural Production Infrastructure and Environment, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece
Aggeliki Rampou - Laboratory of Virology, Scientific Directorate of Phytopathology, Benaki Phytopathological Institute, 8 Stefanou Delta Street, 14561, Athens, Greece
Venetia Psarra - Laboratory of Virology, Scientific Directorate of Phytopathology, Benaki Phytopathological Institute, 8 Stefanou Delta Street, 14561, Athens, Greece
Ioannis Theologidis - Laboratory of Toxicological Control of Pesticides, Scientific Directorate of Pesticides' Control and Phytopharmacy, Benaki Phytopathological Institute, 8 Stefanou Delta Street, 14561, Athens, Greece
Nikon Vassilakos - Laboratory of Virology, Scientific Directorate of Phytopathology, Benaki Phytopathological Institute, 8 Stefanou Delta Street, 14561, Athens, Greece
Détails de publication: Transgenic research, Vol.32(1-2), pp.53-66
Identifiants: 99157291209311
Unité académique: Plant Health Institute of Montpellier - PHIM
Langue: English
Type de ressource: Journal article