Mot1p is essential for TBP recruitment to selected promoters during in vivo gene activation

Jean-Christophe Andrau; Chris J.C. Van Oevelen; Hetty A.A.M. Van Teeffelen; P.Anthony Weil; Frank C.P. Holstege; H.Th.Marc Timmers

doi:10.1093/emboj/cdf485

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Mot1p is essential for TBP recruitment to selected promoters during in vivo gene activation

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Mot1p is essential for TBP recruitment to selected promoters during in vivo gene activation

Jean-Christophe Andrau, Chris J.C. Van Oevelen, Hetty A.A.M. Van Teeffelen, P.Anthony Weil, Frank C.P. Holstege et H.Th.Marc Timmers

The EMBO journal, Vol.21(19), pp.5173-5183

01/10/2002

DOI: https://doi.org/10.1093/emboj/cdf485

PMID: 12356733

Résumé

Recruitment of TATA-binding protein (TBP) is central to activation of transcription by RNA polymerase II (pol II). This depends upon co-activator proteins including TBP-associated factors (TAFs). Yeast Mot1p was identified as a general transcriptional repressor in genetic screens and is also found associated with TBP. To obtain insight into Mot1p function in vivo, we determined the mRNA expression profile of the mot1-1 temperature-sensitive (Ts) strain. Unexpectedly, this indicated that Mot1p mostly plays a positive role for transcription. For one potential activation target, HXT2, we analyzed promoter recruitment of Mot1p, TBP, Taf1p (Taf130p) and pol II by chromatin immunoprecipitation assays. Whereas TBP becomes stably associated upon activation of the HXT2 and HXT4 promoters, Mot1p showed only a transient association. TBP recruitment was compromised in two different mot1 mutant strains, but was only moderately affected in a taf1 Ts strain. Together, our data indicate that Mot1p can assist in recruitment of TBP on promoters during gene activation in vivo.

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Titre: Mot1p is essential for TBP recruitment to selected promoters during in vivo gene activation
Créateurs - sans rôle: Jean-Christophe Andrau - Laboratory for Physiological Chemistry and Department of Medical Genetics, UMC Utrecht, PO Box 85060, 3508 AB Utrecht, The Netherlands, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 and Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA Corresponding author e-mail
Chris J.C. Van Oevelen - Laboratory for Physiological Chemistry and Department of Medical Genetics, UMC Utrecht, PO Box 85060, 3508 AB Utrecht, The Netherlands, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 and Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA Corresponding author e-mail
Hetty A.A.M. Van Teeffelen - Laboratory for Physiological Chemistry and Department of Medical Genetics, UMC Utrecht, PO Box 85060, 3508 AB Utrecht, The Netherlands, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 and Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA Corresponding author e-mail
P.Anthony Weil - Vanderbilt University School of Medicine
Frank C.P. Holstege - Laboratory for Physiological Chemistry and Department of Medical Genetics, UMC Utrecht, PO Box 85060, 3508 AB Utrecht, The Netherlands, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 and Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA Corresponding author e-mail
H.Th.Marc Timmers - Laboratory for Physiological Chemistry and Department of Medical Genetics, UMC Utrecht, PO Box 85060, 3508 AB Utrecht, The Netherlands, Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232 and Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA Corresponding author e-mail
Détails de publication: The EMBO journal, Vol.21(19), pp.5173-5183
Éditeur: Oxford University Press
Nombre de pages: 11
Identifiants: 99139681009311
Unité académique: Institut de Génétique Moléculaire de Montpellier - IGMM
Langue: English
Type de ressource: Journal article