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Monitoring retroviral RNA dimerization in vivo via hammerhead ribozyme cleavage
Journal article   Open access   Peer reviewed

Monitoring retroviral RNA dimerization in vivo via hammerhead ribozyme cleavage

B. K. Pal, L. Scherer, L. Zelby, Edouard Bertrand and J. J. Rossi
Journal of Virology, Vol.72(10), pp.8349--8353
1998
PMCID: PMC110209

Abstract

moloney murine leukemia nucleocapsid protein ncp10 packaging signal viral-rna genomic rna in-vitro virus encapsidation region momulv
We have used a strategy for colocalization of Psi (Psi)-tethered ribozymes and targets to demonstrate that Psi sequences are capable of specific interaction in the cytoplasm of both packaging and nonpackaging cells. These results indicate that current in vitro dimerization models may have in vivo counterparts. The methodology used may be applied to further genetic analyses on Psi domain interactions in vivo.
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https://doi.org/10.1128/JVI.72.10.8349-8353.1998View
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