Modules for C-terminal epitope tagging of Tetrahymena genes

Kensuke Kataoka; Ursula E. Schoeberl; Kazufumi Mochizuki

doi:10.1016/j.mimet.2010.07.009

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Modules for C-terminal epitope tagging of Tetrahymena genes

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Modules for C-terminal epitope tagging of Tetrahymena genes

Kensuke Kataoka, Ursula E. Schoeberl et Kazufumi Mochizuki

Journal of microbiological methods, Vol.82(3), pp.342-346

01/09/2010

DOI: https://doi.org/10.1016/j.mimet.2010.07.009

PMID: 20624430

Résumé

Biochemical Research Methods

Biochemistry & Molecular Biology

Life Sciences & Biomedicine

Microbiology

Science & Technology

Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies. (C) 2010 Elsevier B.V. All rights reserved.

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Détails

Titre: Modules for C-terminal epitope tagging of Tetrahymena genes
Créateurs - sans rôle: Kensuke Kataoka - Austrian Acad Sci IMBA, Inst Mol Biotechnol, A-1030 Vienna, Austria
Ursula E. Schoeberl - Austrian Acad Sci IMBA, Inst Mol Biotechnol, A-1030 Vienna, Austria
Kazufumi Mochizuki - Austrian Acad Sci IMBA, Inst Mol Biotechnol, A-1030 Vienna, Austria
Détails de publication: Journal of microbiological methods, Vol.82(3), pp.342-346
Éditeur: Elsevier
Nombre de pages: 5
Note de subvention: 204986 / European Research Council (ERC) Austrian Academy of Sciences Naito Foundation; Naito Memorial Foundation Austrian Science Fund (FWF) European Community W1207 / Austrian Science Fund (FWF)
Identifiants: 99159291809311
Unité académique: Institut de Génétique Humaine - IGH
Langue: English
Type de ressource: Journal article