Résumé
No effective treatment currently exists for prion diseases and therefore the development of experimental non-human primate models of prion neurotoxicity, to better understand the underlying mechanism and to test new treatments relevant to humans, represents an urgent medical need. However, the establishment of such models is challenging due to animal welfare and cost considerations. We describe here the use of
Microcebus murinus retina, in primary cultures and
in vivo, as a new experimental primate model to rapidly examine the effects in the central nervous system of PrP
106–126, a neurotoxic fragment of the human prion protein. We demonstrate that PrP
106–126 triggered rod photoreceptor cell loss by apoptosis and a change in morphology of microglial cells in mixed neuronal–glial cultures of retinal cells. In addition, 2
days after intravitreal injection of PrP
106–126, retinas showed a significant increase in the number of apoptotic nuclei, mainly in the ganglion cell layer.