Abstract
Microbial biosensors hold great promise for engineering high‐performance, field‐deployable and affordable detection devices for medical and environmental applications. This review explores recent advances in the field, highlighting new sensing strategies and modalities for whole‐cell biosensors as well as the remarkable expansion of microbial cell‐free systems. We also discuss improvements in robustness that have enhanced the ability of biosensors to withstand the challenging conditions found in biological samples. However, limitations remain in expanding the detection repertoire, particularly for proteins. We anticipate that the AI‐powered revolution in protein design will streamline the engineering of custom‐made sensing modules and unlock the full potential of microbial biosensors.