Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part I: Optimisation of derivated histamine detection with coated-plates using optimal design

M. Claeys-Bruno; O. Vandenabeele-Trambouze; M. Sergent; M. Geffard; D. Bodet; M. Dobrijevic; A. Commeyras; R. Phan Tan Luu

doi:10.1016/j.chemolab.2005.06.006

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Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part I: Optimisation of derivated histamine detection with coated-plates using optimal design

Journal article

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Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part I: Optimisation of derivated histamine detection with coated-plates using optimal design

M. Claeys-Bruno, O. Vandenabeele-Trambouze, M. Sergent, M. Geffard, D. Bodet, M. Dobrijevic, A. Commeyras and R. Phan Tan Luu

Chemometrics and Intelligent Laboratory Systems, Vol.80, pp.176-185

2006

DOI: https://doi.org/10.1016/j.chemolab.2005.06.006

Abstract

Experimental design

Hybrid design

Antibodies

Derivatization

Histamine analysis

Using derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (<300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed against histamine, they were characterized and used for ELISA measurements. The development of a quantitative method needs an optimization of both detection and derivatization step. Experimental design methodology has been applied to optimize the conditions of the detection of the derivatized histamine. Methods and results were closely related.

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Title: Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part I: Optimisation of derivated histamine detection with coated-plates using optimal design
Creators - without role: M. Claeys-Bruno - Institut des Sciences Moléculaires de Marseille
O. Vandenabeele-Trambouze - Organisation moléculaire (évolution et matériaux fluores)
M. Sergent - Institut des Sciences Moléculaires de Marseille
M. Geffard - Laboratoire de l'Intégration du Matériau au Système
D. Bodet - Laboratoire de physique des interactions ondes matières
M. Dobrijevic - Observatoire Aquitain des Sciences de l'Univers
A. Commeyras - Organisation moléculaire (évolution et matériaux fluores)
R. Phan Tan Luu - Institut des Sciences Moléculaires de Marseille
Publication Details: Chemometrics and Intelligent Laboratory Systems, Vol.80, pp.176-185
Identifiers: 9935640709311
Academic Unit: Université de Montpellier
Language: English
Resource Type: Journal article
Local Fields: hal-00023303