Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

Juan González-Vera; David Bouzada; Céline Bouclier; M. Eugenio Vázquez; May Morris

doi:10.1039/c6cc09948c

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Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

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Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity

Juan González-Vera, David Bouzada, Céline Bouclier, M. Eugenio Vázquez et May Morris

Chemical Communications, Vol.53(45), pp.6109-6112

2017

DOI: https://doi.org/10.1039/c6cc09948c

Résumé

Coordination complexes / chemistry

Cyclin-Dependent Kinase 4 / analysis

Fluorescent Dyes / metabolism

Heterocyclic Compounds, 1-Ring / chemistry

Peptides / metabolism

Aminopyridines / pharmacology

Benzidimazoles / pharmacology

We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.

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Titre: Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity
Créateurs - sans rôle: Juan González-Vera - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
David Bouzada - Center for Research in Molecular Medicine and Chronic Diseases
Céline Bouclier - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
M. Eugenio Vázquez - Centro de Investigación en Química Biolóxica e Materiais Moleculares
May Morris - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
Détails de publication: Chemical Communications, Vol.53(45), pp.6109-6112
Identifiants: 99153387609311
Unité académique: Institut des Biomolécules Max Mousseron - IBMM
Langue: English
Type de ressource: Journal article
Champs locaux: hal-03032037