Abstract
A huge diversity of oxygenated metabolites originatedfrom polyunsaturated fatty acids (PUFAs) had been discovereddiscovered. Radical cascades are involved in the biosynthesis ofthese metabolites, and the radical initiation can be performed eitherinside the active site of an enzyme in the extracellular medium, or inmembrane on phospholipids. The development of convergent andflexible chemical strategies to prepare these compounds by organicchemists, along with the development of sophisticated massspectrometry methods has boosted the knowledge regarding PUFAmetabolites. Also, isoprostanoids have emerged as indicators ofoxidative stress in humans and their environment. This reviewprovides a brief overview on the chemical strategies and analyticalmethods achieved over the past thirty years.