Abstract
Ten new microsatellite markers were isolatedfrom the freshwater blenny, Salaria fluviatilis. These lociwere characterised for three distinct populations (VarRiver and Lake Bourget in France, and Lake Kinneret inIsrael). S. fluviatilis populations in the North-West of theMediterranean Sea are all polymorphic and under Hardy–Weinberg equilibrium. These microsatellite markers aresuggested for studying the genetic structure in this area.Seven of the ten microsatellite markers are applicable forall the freshwater blenny populations of the entire Mediterraneanbasin. Cross-species characterisation was alsoperformed on the peacock blenny, Salaria pavo. Seven ofthe ten microsatellite loci exhibited successful PCRamplification on this latter species. Polymorphism isobserved on four of these seven loci.