Résumé
Mammals contain a family of five closely related H1 histone variants (H1a-e) as well as two less closely related forms, H1° and H1t. We have sequenced a rat genomic clone that encodes one of the standard H1 variants. An RNA transcript of the gene was made with bacteriophage SP6 RNA polymerase and translated in a cell-free system. The protein synthesized in vitro was identified as variant H1d by its electrophoretic mobility.