Résumé
Sixty five
Xanthomonas albilineans strains originating from 22 countries were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins, indirect-immunofluorescent antibody typing and gas-liquid chromatographic analysis of fatty acid methyl esters (FAMEs). The strains were separated into two major SDS-PAGE protein clusters, three serovars and three FAME groups. SDS-PAGE protein cluster 1 contained 48 strains which represented a world-wide geographic distribution (with the exception of Fiji and tropical Africa), the majority of which (47) belonged to serovar 1. SDS-PAGE protein cluster 2 comprised 17 strains mainly from tropical Africa and Fiji, which belonged either to serovars 2 or 3 (except strain LMG 478). There was less correlation between the grouping based on FAME composition and those based on SDS-PAGE protein profiles or serological properties. The genomic relationship between
X. albilineans and other
Xanthomonas species was investigated by DNA: DNA hybridization. A DNA relatedness of 97% was found within
X. albilineans, whereas low DNA hybridization values (6–37%) were observed between
X. albilineans type strain LMG 494 and representative strains from the other 20
Xanthomonas DNA hybridization groups so far determined. Within the genus
Xanthomonas, X. albilineans clearly constitutes a well-delineated species.