Résumé
Eukaryotic gene transcription is regulated at many steps, including RNA polymerase II (Pol II) recruitment, transcription initiation, promoter-proximal Pol II pause release, and transcription termination; however, mechanisms regulating transcription during productive elongation remain poorly understood. Enhancers, which activate gene transcription, themselves undergo Pol II-mediated transcription, but our understanding of enhancer transcription and enhancer RNAs (eRNAs) remains incomplete. Here we show that transcription at intragenic enhancers interferes with and attenuates host gene transcription during productive elongation. While the extent of attenuation correlates positively with nascent eRNA expression, the act of intragenic enhancer transcription alone, but not eRNAs, explains the attenuation. Through CRISPR/Cas9-mediated deletions, we demonstrate a physiological role for intragenic enhancer-mediated transcription attenuation in cell fate determination. We propose that intragenic enhancers not only enhance transcription of one or more genes from a distance but also fine-tune transcription of their host gene through transcription interference, facilitating differential utilization of the same regulatory element for disparate functions.
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•Intragenic enhancers, besides activating genes, also attenuate host gene expression•Transcription at intragenic enhancers interferes with host gene transcription•The act of enhancer transcription alone, but not the eRNA, explains the attenuation•Intragenic enhancer-mediated attenuation determines cell fate choice
Cinghu et al. report an unanticipated role for intragenic enhancers in attenuating host gene expression. They show that transcription at intragenic enhancers interferes with and attenuates host gene transcription during productive elongation. Genetic experiments reveal a physiological role for intragenic enhancer-mediated attenuation in cell fate choice during embryonic stem cell differentiation.