Résumé
<b>Rationale:</b> During development, the contribution of IL34, a ligand of macrophage colony stimulating factor receptor (MCSFR), has not been fully defined. Together with its twin cytokine MCSF, they display an essential role in macrophage differentiation and activation, including tissue specialized macrophages. The mechanism of action of each molecule involves the phosphorylation of MCSFR in varying intensity and kinetics. Furthermore, IL34 can interact with other receptors and cofactors, opening a wide range of modulations during development. The aim of this work was to investigate these effects through the suppression of IL34 in different animal models and study molecular interactions, with a particular focus on osteoclast / osteoblast regulation.<br /><b>Methods:</b> Two different and unique models of IL34 -/-were generated in zebrafish and mouse. The skeleton of both species was analyzed and compared by histological and morphometric (Micro-CT) approaches. The role of IL34 and new partners in osteoclast and osteoblast differentiation was analyzed by multiple techniques including mineralization assays, tartrate resistant acid phosphatase (TRAP) staining, receptor phosphorylation and activation assays, and gene expression (real-time quantitative PCR) studies. Furthermore, protein interactions were studied by surface plasmon resonance approach and protein-protein docking ClusPro analysis.<br /><b>Results:</b> Significant growth delay and hypo-mineralization of skeletal elements were observed in both IL34 -/-models, as well as craniofacial dysmorphoses in mice. With regard to bone cells, an unexpected increase in the number of osteoclasts and an accumulation of preosteoblasts were observed in mice lacking IL34. For the first time, in vitro analyses complemented by protein binding and molecular docking studies established that IL34 interacts directly with certain Bone Morphogenetic Proteins (BMPs), modulating their various activities such as the stimulation of osteoblast differentiation.<br /><b>Conclusions:</b> A new mechanism of action for IL34 through BMPs has been characterized. IL34 interactions with MCSFR and BMPs appear crucial for both osteoclastogenesis and osteoblastogenesis, impacting bone tissue homeostasis and development. The potential interaction of IL34 with different members of the BMP family and their functional impact, including pathological situations such as cancer, should be further explored, opening new therapeutic perspectives.