Influence of the 20-kDa protein from Bacillus thuringiensis ssp. israelensis on the rate of production of truncated Cry1C proteins

Cécile Rang; Martine Bes; Valérie Lullien-Pellerin; Dong Wu; Brian A. Federici; Roger Frutos

doi:10.1016/0378-1097(96)00232-7

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Influence of the 20-kDa protein from Bacillus thuringiensis ssp. israelensis on the rate of production of truncated Cry1C proteins

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Influence of the 20-kDa protein from Bacillus thuringiensis ssp. israelensis on the rate of production of truncated Cry1C proteins

Cécile Rang, Martine Bes, Valérie Lullien-Pellerin, Dong Wu, Brian A. Federici et Roger Frutos

FEMS microbiology letters, Vol.141(2), pp.261-264

01/08/1996

DOI: https://doi.org/10.1016/0378-1097(96)00232-7

PMID: 8768532

Résumé

Bacillus thuringiensis

Cry1C

Molecular chaperone

Protein stability

The potential role of a molecular chaperone on the rate of production of extensively altered Bacillus thuringiensis Cry 1C proteins was investigated. Analysis of the proteins produced by the recombinant B. thuringiensis strains showed that the truncated proteins were produced at a low rate. Expression of the 20-kDa protein gene from B. thuringiensis ssp. israelensis in tandem with the truncated- cry1C genes led to the production of a greater amount of proteins. The formation of inclusion bodies, however, did not occur even when the 20-kDa protein gene was expressed.

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Détails

Titre: Influence of the 20-kDa protein from Bacillus thuringiensis ssp. israelensis on the rate of production of truncated Cry1C proteins
Créateurs - sans rôle: Cécile Rang - BIOTROP-IGEPAM, CIRAD, 2477 Avenue du Val de Montferrand B.P. 5035, 34032 Montpellier Cedex 1, France
Martine Bes - BIOTROP-IGEPAM, CIRAD, 2477 Avenue du Val de Montferrand B.P. 5035, 34032 Montpellier Cedex 1, France
Valérie Lullien-Pellerin - Unité de Biochimie et Biologie Moléculaire des Céréales, 1NRA, 2 place Viala, 34060 Montpellier Cedex 1, France
Dong Wu - Department of Entomology, University of California, Riverside, CA 92521, USA
Brian A. Federici - Department of Entomology, University of California, Riverside, CA 92521, USA
Roger Frutos - BIOTROP-IGEPAM, CIRAD, 2477 Avenue du Val de Montferrand B.P. 5035, 34032 Montpellier Cedex 1, France
Détails de publication: FEMS microbiology letters, Vol.141(2), pp.261-264
Éditeur: Elsevier B.V
Nombre de pages: 4
Identifiants: 99155004309311
Unité académique: Intéractions hôtes-vecteurs-parasites-environnement maladies tropicales - INTERTRYP
Langue: English
Type de ressource: Journal article