Induction of dnaK through Its Native Heat Shock Promoter Is Necessary for Intramacrophagic Replication of Brucella suis

Stephan Köhler; Euloge Ekaza; Jean-Yves Paquet; Karl Walravens; Jacques Teyssier; Jacques Godfroid; Jean-Pierre Liautard

doi:10.1128/IAI.70.3.1631-1634.2002

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Induction of dnaK through Its Native Heat Shock Promoter Is Necessary for Intramacrophagic Replication of Brucella suis

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Induction of dnaK through Its Native Heat Shock Promoter Is Necessary for Intramacrophagic Replication of Brucella suis

Stephan Köhler, Euloge Ekaza, Jean-Yves Paquet, Karl Walravens, Jacques Teyssier, Jacques Godfroid et Jean-Pierre Liautard

Infection and immunity, Vol.70(3), pp.1631-1634

01/03/2002

DOI: https://doi.org/10.1128/IAI.70.3.1631-1634.2002

PMID: 11854256

Résumé

Cellular Microbiology: Pathogen-Host Cell Molecular Interactions

The heat shock protein DnaK is essential for intramacrophagic replication of Brucella suis. The replacement of the stress-inducible, native dnaK promoter of B. suis by the promoter of the constitutively expressed bla gene resulted in temperature-independent synthesis of DnaK. In contrast to a dnaK null mutant, this strain grew at 37°C, with a thermal cutoff at 39°C. However, the constitutive dnaK mutant, which showed high sensitivity to H2O2-mediated stress, failed to multiply in murine macrophage-like cells and was rapidly eliminated in a mouse model of infection, adding strong arguments to our hypothesis that stress-mediated and heat shock promoter-dependent induction of dnaK is a crucial event in the intracellular replication of B. suis.

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Titre: Induction of dnaK through Its Native Heat Shock Promoter Is Necessary for Intramacrophagic Replication of Brucella suis
Créateurs - sans rôle: Stephan Köhler - Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France, Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Center, B-1180 Brussels, Belgium
Euloge Ekaza - Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France, Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Center, B-1180 Brussels, Belgium
Jean-Yves Paquet - Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France, Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Center, B-1180 Brussels, Belgium
Karl Walravens - Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France, Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Center, B-1180 Brussels, Belgium
Jacques Teyssier - Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France, Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Center, B-1180 Brussels, Belgium
Jacques Godfroid - Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France, Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Center, B-1180 Brussels, Belgium
Jean-Pierre Liautard - Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France, Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Center, B-1180 Brussels, Belgium
Détails de publication: Infection and immunity, Vol.70(3), pp.1631-1634
Éditeur: American Society for Microbiology
Nombre de pages: 4
Identifiants: 99155343209311
Unité académique: Institut de Recherche en Infectiologie de Montpellier - IRIM
Langue: English
Type de ressource: Journal article