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In vivo formaldehyde cross-linking: it is time for black box analysis
Article de revue

In vivo formaldehyde cross-linking: it is time for black box analysis

Alexey Gavrilov, Sergey V. Razin et Giacomo Cavalli
Briefings in functional genomics, Vol.14(2), pp.163-165
01/03/2015
PMID: 25241225

Résumé

Biotechnology & Applied Microbiology Genetics & Heredity Life Sciences & Biomedicine Science & Technology
Formaldehyde cross-linking is an important component of many technologies, including chromatin immunoprecipitation and chromosome conformation capture. The procedure remains empirical and poorly characterized, however, despite a long history of its use in research. Little is known about the specificity of in vivo cross-linking, its efficiency and chemical adducts induced by the procedure. It is time to search this black box.

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