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In vitro infection of primary human hepatocytes by HCV-positive sera: insights on a highly relevant model
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In vitro infection of primary human hepatocytes by HCV-positive sera: insights on a highly relevant model

Claire Gondeau, Philippe Briolotti, Francia Razafy, Cédric Duret, Pierre-Alain Rubbo, Francois Helle, Thierry Rème, Marie-Pierre Ripault, Jacques Ducos, Jean-Michel Fabre, …
Gut, Vol.63(9), pp.1490-1500
03/08/2014
PMID: 24153249

Résumé

HEPATOCYTE HEPATITIS C CYTOKINES CHRONIC HEPATITIS CHEMOKINES Adult Biomarkers Models, Immunological Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Serum Cell Culture Techniques Cell Line Cells, Cultured Cytokines Hepacivirus Hepatocytes Humans Kinetics
Adult primary human hepatocytes (PHHs) support the complete infection cycle of natural HCV from patients' sera. The molecular details underlying sera infectivity towards these cells remain largely unknown. Therefore, we sought to gain a deeper comprehension of these features in the most physiologically relevant culture system.

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