INITIAL TRANSCRIPTION BY RNA POLYMERASE PROCEEDS THROUGH A DNA-SCRUNCHING MECHANISM: Single-molecule fluorescence-resonance-energy-transfer experiments establish that initial transcription proceeds through a “scrunching” mechanism, in which RNA polymerase remains fixed on promoter DNA and pulls downstream DNA into itself and past its active center

Achillefs N. Kapanidis; Emmanuel Margeat; Sam On Ho; Ekaterine Kortkhonjia; Shimon Weiss; Richard H. Ebright

doi:10.1126/science.1131399

Retour

INITIAL TRANSCRIPTION BY RNA POLYMERASE PROCEEDS THROUGH A DNA-SCRUNCHING MECHANISM: Single-molecule fluorescence-resonance-energy-transfer experiments establish that initial transcription proceeds through a “scrunching” mechanism, in which RNA polymerase remains fixed on promoter DNA and pulls downstream DNA into itself and past its active center

Article de revue

Avec comité de lecture

INITIAL TRANSCRIPTION BY RNA POLYMERASE PROCEEDS THROUGH A DNA-SCRUNCHING MECHANISM: Single-molecule fluorescence-resonance-energy-transfer experiments establish that initial transcription proceeds through a “scrunching” mechanism, in which RNA polymerase remains fixed on promoter DNA and pulls downstream DNA into itself and past its active center

Achillefs N. Kapanidis, Emmanuel Margeat, Sam On Ho, Ekaterine Kortkhonjia, Shimon Weiss et Richard H. Ebright

Science (American Association for the Advancement of Science), Vol.314(5802), pp.1144-1147

17/11/2006

DOI: https://doi.org/10.1126/science.1131399

PMID: 17110578

Résumé

Using fluorescence resonance energy transfer to monitor distances within single molecules of abortively initiating transcription initiation complexes, we show that initial transcription proceeds through a “scrunching” mechanism, in which RNA polymerase (RNAP) remains fixed on promoter DNA and pulls downstream DNA into itself and past its active center. We show further that putative alternative mechanisms for RNAP-active-center translocation in initial transcription, involving “transient excursions” of RNAP relative to DNA or “inchworming” of RNAP relative to DNA, do not occur. The results support a model in which a stressed intermediate, with DNA-unwinding stress and DNA-compaction stress, is formed during initial transcription, and in which accumulated stress is used to drive breakage of interactions between RNAP and promoter DNA and between RNAP and initiation factors during promoter escape.

Indicateurs

1 Consultations de la notice

Détails

Titre: INITIAL TRANSCRIPTION BY RNA POLYMERASE PROCEEDS THROUGH A DNA-SCRUNCHING MECHANISM
Créateurs - sans rôle: Achillefs N. Kapanidis - Department of Chemistry and Biochemistry and Department of Physiology, University of California, Los Angeles, CA 90095, USA Clarendon Laboratory, Department of Physics, and IRC in Bionanotechnology, University of Oxford, Oxford, United Kingdom Howard Hughes Medical Institute, Department of Chemistry, and Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
Emmanuel Margeat - Department of Chemistry and Biochemistry and Department of Physiology, University of California, Los Angeles, CA 90095, USA Clarendon Laboratory, Department of Physics, and IRC in Bionanotechnology, University of Oxford, Oxford, United Kingdom Howard Hughes Medical Institute, Department of Chemistry, and Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
Sam On Ho - Department of Chemistry and Biochemistry and Department of Physiology, University of California, Los Angeles, CA 90095, USA Clarendon Laboratory, Department of Physics, and IRC in Bionanotechnology, University of Oxford, Oxford, United Kingdom Howard Hughes Medical Institute, Department of Chemistry, and Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
Ekaterine Kortkhonjia - Department of Chemistry and Biochemistry and Department of Physiology, University of California, Los Angeles, CA 90095, USA Clarendon Laboratory, Department of Physics, and IRC in Bionanotechnology, University of Oxford, Oxford, United Kingdom Howard Hughes Medical Institute, Department of Chemistry, and Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
Shimon Weiss - University of California, Los Angeles
Richard H. Ebright - Department of Chemistry and Biochemistry and Department of Physiology, University of California, Los Angeles, CA 90095, USA Clarendon Laboratory, Department of Physics, and IRC in Bionanotechnology, University of Oxford, Oxford, United Kingdom Howard Hughes Medical Institute, Department of Chemistry, and Waksman Institute, Rutgers University, Piscataway, NJ 08854, USA
Détails de publication: Science (American Association for the Advancement of Science), Vol.314(5802), pp.1144-1147
Identifiants: 99149394109311
Unité académique: Centre de Biologie Structurale - CBS
Langue: English
Type de ressource: Journal article