Résumé
Uric acid (UA), the terminal metabolite of purine catabolism, is a critical biomarker requiring precise monitoring. Conventional detection methods (e.g., enzymatic assays, HPLC) suffer from limited sensitivity and operational complexity. To address this, we engineered four colloidal SERS substrates (Ag NPs, AuAg NPs, Au NPs, Au NRs) for signal amplification and fabricated hydrophobic surfaces via hydrochloric acid etching and evaporative deposition of heptadecafluorodecyl trimethoxysilane to minimize analyte dispersion. Integration of optimized Ag NPs with the hydrophobic substrate enabled quantitative UA detection by SERS. This approach achieves rapid (∼1 min), demonstrating significant potential for point-of-care UA monitoring.
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•Hydrophobic substrates via acid etching and silane evaporation inhibit sample diffusion for enhanced SERS detection.•Various metal nanoparticles were synthesized and tested, with Ag NPs showing the best enhancement for uric acid detection.•A handheld Raman spectrometer was integrated with the hydrophobic substrate enables rapid, on-site uric acid detection.•The method achieved quantitative detection of uric acid with a linear range from 10−3 M to 10−5 M.