Résumé
The action of environmental chemicals (ECs) on the mineralocorticoid receptor (MR) has beensuggested to impair physiological processes regulated by this nuclear receptor. However, it remainsunderstudied both as target of ECs and with respect to potential species-specific differences. In thisregard, we have developed reporter cell lines to identify the response to different steroids, ECs andurban wastewater (WW) sample extracts of human MR (hMR) and zebrafish MR (zfMR). Most of thesteroids had a higher efficacy on zfMR than hMR while the ECs were antagonists to both hMR andzfMR, with a lower potency on the latter. Interestingly, WW sample extracts revealed the presence ofMR activity with a greater activity on zfMR compared to hMR, suggesting the presence of steroids inWW. These screening tools have proven to be powerful tools for characterizing the interaction ofchemicals with MRs and revealing their presence in environmental samples.